Development of a fluorescein analogue, TokyoMagenta, as a novel scaffold for fluorescence probes in red region.
نویسندگان
چکیده
We present a design strategy for fluorescence probes with a high off/on activation ratio in the red wavelength region, based on a novel fluorescein analogue in which the O atom at the 10 position of the xanthene chromophore is replaced with a Si atom. To demonstrate the usefulness of this strategy, we designed and synthesized a red-fluorescent probe for β-galactosidase, and showed that it works in live HEK293 cells.
منابع مشابه
Red fluorescent probe for monitoring the dynamics of cytoplasmic calcium ions.
The development of sophisticated fluorescent probes has contributed to the elucidation of the molecular mechanisms of many complex biological phenomena. In particular, fluorescence imaging of the calcium ion (Ca) has become an essential technique for the investigation of signaling pathways involving Ca as a second messenger. For example, changes in the intracellular Ca concentration have been f...
متن کاملDevelopment of practical red fluorescent probe for cytoplasmic calcium ions with greatly improved cell-membrane permeability.
Fluorescence imaging of calcium ions (Ca(2+)) has become an essential technique for investigation of signaling pathways involving Ca(2+) as a second messenger. But, Ca(2+) signaling is involved in many biological phenomena, and therefore simultaneous visualization of Ca(2+) and other biomolecules (multicolor imaging) would be particularly informative. For this purpose, we set out to develop a f...
متن کاملآشکارسازی مواد منفجره نیتروآروماتیکی با استفاده از ترکیبات فلوئورسنت (علمی-پژوهشی)
Recently almost all of the analytical methods have been used for analyzing and detecting of explosives, because of their importance in demining and unexploded ordnances operations. Fluorescence technique is one of the analytical methods. The unique features of this method caused to lots of researches in the mentioned applications. In present study, the fluorophores such as pyrene, phenol red, m...
متن کاملApplication of FITC for detecting the binding of antiangiogenic peptide to HUVECs
Angiogenesis is the generation of new blood vessels from the existing vasculature. The angiogenic programme requires the degradation of the basement membrane, endothelial cell migration and invasion of the extracellular matrix, with endothelial cell proliferation and capillary lumen formation before maturation and stabilization of the new vasculature. Angiogenesis is dependent on a delicate equ...
متن کاملSemiconductor nanocrystal probes for human metaphase chromosomes.
To improve signal stability and quantitation, an optically stable, novel class of fluorophore for hybridization analysis of human metaphase chromosomes is demonstrated. Detection of hybridization sites in situ was based on fluorescence from streptavidin-linked inorganic crystals of cadmium selenide [(CdSe)ZnS]. Fluorescence of nanocrystal fluorophores was significantly brighter and more photost...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Chemical communications
دوره 47 14 شماره
صفحات -
تاریخ انتشار 2011